pcr amplification造句

• The length of the 2nd intron sequences obtained by pcr amplification ranges from 153 to 168 bp in the 11 sisorid species ( 17 samples ) investigated , and is 154 bp in liobagrus kingi and liobagrus marginatoides
  通过pcr技术，扩增了长度为154bp的金氏(鱼央)和拟缘(鱼央)以及长度为153 168bp的11种?科鱼类( 17个样品)的rps7基因内含子2序列。
• After the second pcr amplification , 10 differential cd na fragments were identified among which 6 fragments were wild type specific and 4 fragments were ast mutant specific . all of the 10 differential cdna fragments were sequenced
  二次pcr扩增后，进一步筛选出10个差异表达的cdna条带，其中6个是野生型特异表达的， 4个是突变型特异表达的。
• Pcr amplification of hla - dqa1 use the extracted genome dna as template , amplify the target sequence . examine the products by page . the bands is clear and the length is 538bp as it shows , which matches the design
  结果1 . hla一dqai基因片段的pcr扩增以提取的全基因组dna为模板，行pcr扩增，产物用聚丙烯酞胺凝胶电泳检测，可见条带清晰，产物长度538bp ，与设计相符。
• Pcr amplification of the target sequence design a pair of primers ac - coding to the conservative region of hla - dqa1 exon 2 , and lable the sence strand by fitc . make the fiuorescenced strand the advantage strand by assym - metric pcr . 3
  样本靶序列的pcr扩增在hla - dqa1第二外显子保守区域设计一对引物，并在正义链引物5 ’作fitc标记，以荧光标记单链为优势链，行不对称扩增。
• Liobagrus kingi and liobagrus marginatoides were designated as outgroups in the present study . the length of 16s rrna gene fragment obtained by pcr amplification ranges from 534 to 542 bp in the 11 sisorid species ( 31 samples ) investigated , and is 532 bp in l
  通过pcr技术，扩增了长度为532bp的金氏(鱼央)和拟缘(鱼央)以及长度为534 - 542bp的11种?科鱼类( 31个样品)的16srrna基因片段。
• Finally , the variant line tc243 - 2 , was analyzed by rapd - pcr amplification with 100 random primers . the results suggested that phenotypic variation came from exogenous dna transformation rather than exogenous pollen contamination . a mutation corre
  用100条随机引物对tc243一株系的rapd一pcr扩增结果表明该株系的变异不是来自外源花粉的污染，同时发现了特异条带5270 _ 1600对应的变异在tz一ts代能连续稳定遗传。
• Experiment shows that tpsl gene can endow organism the ability of synthesis trehalose , the dephosphorylation of the trehalose - 6 - phosphate is not special , and it can be replaced by other phosphatases . the tpsl gene from saccharomyces cerevisiae was cloned by pcr amplification
  实验证实tps1基因就可以使生物体获得产生海藻糖的能力，酵母的海藻糖合成酶复合体中6 -磷酸-海藻糖的脱磷酸化作用是非特异性的，它可由生物体内的其它酯酶所代替。
• The room for the following equipments need the air - conditioning : uv - spectrophotometer , fluorescence spectrometer , gc , gc - ms , aas , afs , icp , icp - ms , hplc , hplc - ms , ion chromatography , atomic fluores - cence spectrometer , pcr amplification and balance
  12紫外/可见分光光度仪、荧光分光光度，气相色谱，气相色谱质谱、原子吸收，原子荧光仪、等离子发射光谱、等离子质谱、高效液相、液相色谱质谱、离子色谱、荧光光度、 pcr核酸扩增仪以及天平室安装空调。
• A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification . the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too . then the outcome was transformed into escherichia coli bl21
  Pcr产物经nde / ecor双酶切后，与同样双酶切的pet - 30a ( + )质粒连接并转化至大肠杆菌( escherichiacoli ) bl21中，经检测筛选，成功得到阳性克隆。
• Among the three methods used in the experiment of dna extraction , only ctab , adding pvp in the dna isolation step , had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process . pcr amplifications were performed in a final volume of 25 mm3 containing 0 . 5 units taq polymerase , 2
  通过在抽提缓冲液加入2的pvp 、并用异丙醇和乙醇沉淀基因组dna等改良措施， ctab法能避开大量纤维、多糖的影响，有效地从不同属种的棕榈科植物的幼嫩叶片中提取并纯化了适合rapd的基因组dna 。
• In order to clone new genes expressed during early embryonic development of trionyx sinensis , we constructed and characterized a cdna expression library from poly ( a + ) mrna isolated from 250 mg of cranial / kidney / gonad complex tissues of one - week - old embryos of trionyx sinesis using the smart ( switching mechanism at 5 " end of rna transcript ) cdna synthesis and ld - pcr amplification strategy
  为了克隆到与胚胎发育有关的新基因，以孵化一星期的中华鳖( trto尸砂优sinensis )胚胎的头部、生殖晴混合组织为原始材料，采用smart但witching丛eehanism丛5 ’ endof旦nairanseript )和长距离pcr伍d一pcr )技术，构建t一个中华鳖cdna表达文库。
• The gpa1 gene was obtained via pcr amplification and was cloned in the two - hybrid dna binding domain vector pgbkt7 the combinant plasmid was designated as pgbkt7 - ga . - galactosidase assay indicated that got did not have the property of self - activation . after pgbkt7 - ga was transformed to yeast pj69 - 4a , we transformed arabidopsis vegetative tissue two - hybrid cdna library plasmids to yeast pj69 - 4a containing pgbkt7 - ga
  通过pcr扩增得到gpa1基因并将其克隆到双杂交dna结合域载体pgbkt7中，得到的重组质粒命名为pgbkt7 - g ， ?半乳糖苷酶活性鉴定表明g不具有自激活特性，将其转化到酵母菌pj69 - 4a中，再以此为受体菌转化拟南芥绿色营养组织cdna文库质粒。
• Total rna was extracted from pituitary of new butchered female goat for fsh - a and b subunit genes . cdna were synthesized by rt - pcr reactions respectively and these cdna were used as templates in pcr amplifications . the pcr products were 360bp and 390bp which just were the same length of the predicted goat fsh - a and p subunit genes
  本试验是从新屠宰的母山羊脑垂体中提取总rna ，反转录获得cdna ，以此cdna为模板， pcr扩增目的基因片段，分别获得长为360bp的山羊fsh -亚基dna片段和长为390bp的山羊fsh -亚基dna片段，与预期的目的基因片段大小一致。
• ( 2 ) deletions of four different lengths were conducted using pcr amplification with the information of sequence of pdf 1 . 2 gene promoter . these mutated promoters were fused to the gus reporter gene and introduced into tobacco plants . the results of gus activity driven by these different constructs showed that the promoter sequence between positions - 300 and - 243 ( containing a gcc box and a g box - like sequence ) was an essential ja - responsive element region to activate the expression of gus reporter gene , and the - 300 bp position was defined as the boundary of the minimal functional promoter in response to ja signaling
  ( 2 )对该启动子的5 ’端进行不同长度的缺失突变，突变的启动子与gus构建的融合基因在烟草中受meja诱导的瞬时表达结果表明，该启动子中- 300 - 243bp区域(有一个gccbox和一个gbox - likesequence )是其应答meja处理所必须的区域，并将- 300bp作为该启动子应答ja信号的最小区域的边界位置。
• The hemaglutinin ( ha ) of aiv plays the key role in determing the pathogenicity , cell receptor binding property and host range of the virus . the homology of the ha sequences reported and registered in genbank of different strains of h _ ( 5 ) . h _ ( 9 ) subtype was respectively analyzed and compared with each other . the conservative domin of ha seqence of h _ ( 5 ) , h _ ( 9 ) subtype was selected for pcr amplification . two sets of specific primers were designed
  本研究根据genbank中查出的禽流感病毒h _ 5亚型，禽流感病毒h _ 9亚型的ha片段基因组序列，利用dnasis软件分别对aiv的h _ 5 、 h _ 9亚型ha基因区域进行同源性比较，设计筛选出两对联合pcr反应的特异性引物，其中一对是h _ 9亚型通用引物，扩增出ha片段695bp ，另一对引物为h _ 5亚型的通用引物，扩增出的ha区域部分目的片段约为448bp 。
• The probe detection and pcr amplification are contemporary , without ethidium bromide staining and gel running and any post - pcr manipulations , so that the contamination risks are reduced . those two methods were used to detect successfully the primus necrotic ringspot virus from sample delivered by beijing airport quarantine bureau . the amplified fragment was cloned into the vector of pmd18 - t then sequenced
  我们利用建立的杂交诱捕们pcr elisa和队f pcr对北京机场检疫局送检的樱桃样品检出了pnrsv ，同时把451hp的pcr产物克隆到ppo18 t载体上，通过序列测定，同源世最高的是genebank中pnrsv资料序号gi15750502gb山57046
• Putative transgenic plants were screened b y nptii - specific and mnsod - specific pcr amplification and southern blotting , 84 % of the transgenic plants gave positive results . the results of mnsod activities demonstrated that tobacco mnsod gene contributed to more than 50 % of the total mnsod activity in some transgenic alfalfa
  Npt基因和mnsod基因的pcr检测和southern杂交表明mnsod基因已经整合到84的保定苜蓿转基因植株的基因组中， mnsod活性测定结果表明转基因植株中的mnsod活性与对照植株之间存在显著差异，部分转基因植株的mnsod活性比对照植株提高了1倍以上。
• By the same method , the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion . after that , the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method . the pcr amplification indicated the lba4404 strain containning cpti gene . the lba4404 strain was used in genie transformation of mustard
  经酶切和pcr扩增验证后，以xbai和saci双酶切pgem ? cp和pbi121 ，将切下的cpti片段和pbi121载体片段连接构建成表达载体pbi121 - cp ，用冻融法导入农杆菌lba4404 ，提取质粒，经pcr扩增检测，用于芥菜的遗传转化。
• Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates . the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2 , w5 t1 and t7 . these pcr amplified fragments were cloned , and sequenced
  首先利用芽孢杆菌中芽孢的抗热性将土壤溶液在100沸水中煮10 - 15分钟，然后用选择性无氮培养基进行初筛得到29株菌落形态不同的菌株；接着用固氮酶结构基因nifh的特异性引物对这29株菌进行pcr扩增，结果表明其中7个菌株具有nifh基因，这7个菌株的编号依次为： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
• Primers were designed according to the sequences of atcal , bocal , bobcal and boical gene , and pcr amplifications were performed with the genomic dna of brussel sprouts , kale and kohlrabi respectively , pcr fragments of about 1 . 6kb were obtained and designated correspondingly as bogcal5 " , boacal5 " and boccal5 "
  根据拟南芥atcal 、结球甘蓝bocal 、花椰菜bobcal和青花菜boical基因设计引物，对芸薹属植物抱子甘蓝、羽衣甘蓝和球茎甘蓝的基因组dna进行pcr扩增，均得到一段约1 . 6kb大小的pcr产物，分别命名为bogcal5 ’ 、 boacal5 ’和boccal5 ’ 。